Application

Research Sub CategoryNeuroinflammation & PainHormones & Receptors

Immunohistochemistry: 1:100-1:400 on 10 µm cryostat sections of rat and guinea-pig brain stem using fluorescently labled secondary antibodies for detection (Cuello, 1984; MacMillan and Cuello, 1986).

Immunocytochemistry: 1:300 on primary cultures of rat striatal and hippocampal neurons using a Cy2 or Cy3 conjugated secondary antibody for detection (Saudou, 1998).

Radioimmunoassay (Cuello, 1984)

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Detect Enkephalin using this Anti-Enkephalin Antibody, clone NOC1 validated for use in IC, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Leu5-enkephalin conjugated to BSA.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Lyophilized Supernatant, preservative free.

Specificity

Met5- and Leu5-enkephalin. Displays about 40% cross-reactivity with C-terminal extended Met-enkephalin hexapeptides and 7% cross-reactivity with the extended heptapeptide (-Arg-Phe-OH) but does not recognize pro-enkephalin or other endogenous peptides (Cuello, 1984). By immunohistochemistry the antibody lables all sites that are known to exppress enkephalin and shows no cross-reactivity to beta-endorphin or dynorphin (Cuello, 1984; MacMillan and Cuello, 1986).

Storage and Stability

Lyophilized antibody can be kept at 2-8°C for up to 3 months and should be kept at -20°C for long term storage. Reconstitute with 100µL sterile DI water. Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.